Single molecule fluorescence study of the Bacillus thuringiensis toxin Cry1Aa reveals tetramerization

N Groulx, H McGuire, R Laprade, JL Schwartz… – Journal of Biological …, 2011 – ASBMB
… Fluorescence was measured using an Axiovert 200 inverted microscope (Zeiss). A
30-milliwatt 532-nm laser (World Star Tech) was selected as the excitation source.
Excitation light (<1.5 milliwatt) was defocused to a diameter of ≈10 mm. ...